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In,,,addition,,,,the,,,high,,,activity,,,rate,,,,good,,,stability,,,,low,,,cost,,,and,,,wide,,,availability,,,of,,,substrates,,,make,,,HRP,,,the,,,enzyme,,,of,,,choice,,,for,,,most,,,applications.,,,The,,,,vitamin,,,,D,,,,receptor,,,,(VDR),,,,is,,,,expressed,,,,in,,,,skeletal,,,,muscle,,,,of,,,,male,,,,mice,,,,and,,,,modulates,,,,25-hydroxyvitamin,,,,D,,,,(25OHD),,,,uptake,,,,in,,,,myofibers.,,,,The,,,,two,,,,most,,,,commonly,,,,employed,,,,enzymes,,,,are,,,,alkaline,,,,phosphatase,,,,(AP),,,,(Bronstein,,,,etal.,,,,,1989),,,,and,,,,HRP,,,,(Kricka,,,,,1991).,,,,Protein,,,purification.,,,Colorimetric,,detection[edit].,,J,,,Anim,,,Sci,,,1995:,,,73(4):,,,1064–1073.CrossRef,,,,,,PubMed,,,,,,Web,,,of,,,Science,,,Times,,,Cited:,,,112Huyer,,,G,,,,Liu,,,S,,,,Kelly,,,J,,,,Moffat,,,J,,,,Payette,,,P,,,,Kennedy,,,B,,,,Tsaprailis,,,G,,,,Gresser,,,MJ,,,,Ramachandran,,,C.,,,This,,,,gives,,,,researchers,,,,and,,,,corporations,,,,huge,,,,advantages,,,,in,,,,terms,,,,of,,,,flexibility,,,,,and,,,,adds,,,,an,,,,amplification,,,,step,,,,to,,,,the,,,,detection,,,,process.,,,,Eur,,,J,,,Endocrinol,,,2001:,,,145:,,,785–790.CrossRef,,,,,,PubMed,,,,,,CAS,,,,,,Web,,,of,,,Science,,,Times,,,Cited:,,,37Mollica,,,JP,,,,Oakhill,,,JS,,,,Lamb,,,GD,,,,Murphy,,,RM.,,,^,,,a,,,b,,,Towbin,,,H,,,,Staehelin,,,T,,,,Gordon,,,J,,,(1979).,,,The,,,stained,,,bands,,,then,,,indicate,,,the,,,proteins,,,to,,,which,,,the,,,patient's,,,serum,,,contains,,,antibody.,,,Proteins,,,,that,,,,have,,,,been,,,,separated,,,,can,,,,be,,,,cut,,,,out,,,,of,,,,the,,,,gel,,,,and,,,,then,,,,analysed,,,,by,,,,mass,,,,spectrometry,,,,,which,,,,identifies,,,,the,,,,protein.,,,,However,,,,since,,,MCU,,,and,,,MICU1,,,are,,,found,,,within,,,all,,,major,,,eukaryotic,,,taxa,,,,with,,,lineage,,,specific,,,losses,,,,EMRE,,,is,,,unique,,,amongst,,,human,,,uniplex,,,components,,,in,,,that,,,it,,,appears,,,to,,,have,,,emerged,,,more,,,recently,,,and,,,represents,,,a,,,metazoan,,,innovation.,,,If,,,PBS,,,is,,,to,,,be,,,used,,,for,,,intermediate,,,steps,,,,the,,,membrane,,,should,,,be,,,sufficiently,,,washed,,,in,,,TBST,,,to,,,remove,,,excess,,,sodium,,,phosphate,,,before,,,addition,,,of,,,the,,,substrate.It,,,is,,,not,,,only,,,the,,,choice,,,of,,,blocking,,,agent,,,that,,,can,,,affect,,,WB,,,outcomes;,,,volume,,,of,,,blocking,,,agent,,,and,,,indeed,,,the,,,incubation,,,period,,,for,,,blocking,,,(which,,,may,,,vary,,,from,,,an,,,hour,,,to,,,overnight;,,,Gershoni,,,&,,,Palade,,,,1983),,,are,,,also,,,important,,,factors.,,,Oncogene,,2008:,,27(6):,,811–822.CrossRef,,,,PubMed,,,,CAS,,,,Web,,of,,Science,,Times,,Cited:,,31Erickson,,PF,,,Minier,,LN,,,Lasher,,RS.,,A,,wide,,variety,,of,,labeled,,secondary,,detection,,reagents,,can,,be,,used,,for,,Western,,blot,,detection.,,For,,,,example,,,,,the,,,,addition,,,,of,,,,LY294002,,,,to,,,,cultured,,,,cells,,,,inhibits,,,,PI(3)K,,,,,resulting,,,,in,,,,decreased,,,,phosphorylation,,,,of,,,,down-stream,,,,intermediates,,,,(i.e.,,,,,AKT/P70,,,,S6K1),,,,(Rommel,,,,etal.,,,,,2001).,,,,These,,,,are,,,,required,,,,to,,,,be,,,,from,,,,the,,,,same,,,,blots,,,,as,,,,the,,,,other,,,,representative,,,,images,,,,and,,,,should,,,,not,,,,be,,,,repeated,,,,unless,,,,the,,,,same,,,,gel,,,,has,,,,been,,,,stripped,,,,and,,,,reprobed,,,,for,,,,multiple,,,,targets,,,,or,,,,different,,,,molecular-weight,,,,targets,,,,were,,,,measured.,,,, ea41d647aa

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